Transglutaminase Labelling

Directed Protein Modification
Modified proteins are generally utilized in

	Fluorescence-based HTS-assays
	Diagnostics (antibodies, antibody-bindingproteins, lectins etc.)
	Therapeutic proteins (PEGylation)
	Protein immobilization (after biotinylation)

In contrast to chemically labelled proteins transglutaminase labelling leads to specified products characterized by

	Homogeneity
	Defined degree of labelling
	Defined label position(s)
	Minimized amounts of unlabelled protein
	Bioactivity equal to the unlabelled protein
	High solubility in water

Requirements of the target Protein

	Accessible lysine residues or
	Accessible glutamine residues otherwise
	Transglutaminase substrate sequence tags can be introduced recombinantly, if no lysine or glutamin residues are accessible

Principle of TG-catalyzed protein labelling

Available fluorescent labels

Label by yourself using the products listed next side or take advantage of Zedira's Transglutaminase labelling-Service

Step 1
Zedira will first test your target protein's substrate properties.

Step 2
Zedira will optimize the reaction parameters.
Zedira delivers the specified product in a sufficient amount that enables you to test the quality in your assay system.

Step 3
You place an order to produce larger amounts of the labelled protein.