Deamidated Gliadin

Monoclonal antibody to deamidated Gliadin

Quantity Unit Price Status
100 µg 780 € Available
Documents
Art. No.
A160
Background info
Tissue transglutaminase (TG2) catalyzes gliadin deamidation in the intestinal mucosa of celiac disease patients, resulting in deamidated gliadin peptides which are recognized by HLA receptors (DQ2/DQ8) of immune cells. Antibodies to deamidated gliadin have been proven to be specific for celiac disease – in contrast to gliadin antibodies. Detection of deamidated gliadin antibodies now is used in celiac disease diagnostics.
Host
Mouse
Clone
ZDG-01
Isotype
IgG2a/k
Immunogen
Deamidated gliadin-related peptide Lys57-Glu65-[α-gliadin (58-73)] (KLQPFPQPELPYPQPQ).
Specificity
Recognizes Gliadin. Reacts with the deamidated gliadin peptide (KLQPFPQPELPYPQPQ).
Weakly cross-reacts (5%) with the non-deamidated gliadin peptide (KLQPFPQPQLPYPQPQ).
Appearance
liquid, 100 μL with ~ 1 mg/mL
Description
The antibody is supplied as a liquid in PBS, pH 7.4
Application
Suitable for use in ELISA and Western Blot. Other applications not tested.
Working dilutions
Optimal dilutions should be determined by the end user.
The following are guidelines only:
ELISA: 1 / 64,000
Western-Blot: 1 / 1,000
Storage
Stable for short term at + 4 °C
For extended periods: store aliquots at – 20 °C
Reference(s)
Skovbjerg et al., Biochim. Biophys. Acta. 2004, 1690, 220-30
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

News

Blog

Events