Transglutaminase 2

Monoclonal antibody to human TG2 (Beta Sheet Domain, clone XTG11)

(antibody reacts with the Beta Sheet Domain of human transglutaminase 2)
Quantity Unit Price Status
200 µg 400 € Available
Documents
Art. No.
A034
Background info
Tissue transglutaminase is a, Ca2+-dependent enzyme (78 kDa) composed by 4 domains:
Beta Sheet Domain (fibronectin binding, ~17 kDa), catalytic Core Domain (Cys-His-Asp catalytic triad, Calcium-binding, GTP/GDP-binding, ~37 kDa), Beta Barrel 1 Domain (GTP/GDP-binding, ~14 kDa) and Beta Barrel 2 Domain (~12 kDa). The inactive GTP-bound enzyme is present in a closed conformation, which upon activation by Ca2+ and substrate binding opens like a pocket knife resulting in a longitudinal open conformation.
Host
Mouse
Immunogen
Human tissue transglutaminase (full length protein with N-terminal hexahistidin-tag) recombinantly produced in insect cells
Specificity
Mouse monoclonal antibody reacts with the Beta Sheet Domain of TG2.
Specificity of A034 was determined in western blotting with human transglutaminases (TG1 – TG7, FXIII), TG2 of different species and human TG2 domains recombinantly produced in E. coli.
A034 is specific for the Beta-Sheet Domain of TG2. It does not cross-react with other domains of human TG2.
A034 is specific for TG2. It does not cross-react with other human transglutaminases.
A034 is specific for human TG2. It does not cross-react with other TG2 from various species (human, guinea pig, rat, mouse and dog).

A034 recognizes the epitope R76FPLRDAV83 (see figure in PDS). The epitope was determined using PEPperCHIP® Transglutaminase Microarray (Art. No. P111).

For more information klick here:
Poster presentation at GRC, 2016 (Girona, Spain)
Appearance
liquid
Description
Protein G-affinity purified mouse monoclonal antibody.
Reagents
50 mM PBS + 0.01% Na azide, 50% glycerol.
Application
Western-Blotting, Immunofluorescence
Working dilutions
Optimal dilutions should be determined by the end user.
E. g. for Western-Blotting: 1 / 500 to 1 / 5,000 should be suitable
Storage
Store at -80°C.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Stable for short term at +4°C.
Delivery is possible at ambient temperature.
Reference(s)
Kanchan et al., Cell. Mol. Life Sci. 2015, 72:3009-35
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.

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