Transglutaminase 2

Monoclonal antibody to human TG2 (Catalytic Domain, clone XTG17)

(antibody reacts with the Core Domain of transglutaminase 2
[human, guinea pig, rat, mouse and dog TG2])
Quantity Unit Price Status
200 µg 400 € Available
Documents
Art. No.
A033
Background info
Tissue transglutaminase is a, Ca2+-dependent enzyme (78 kDa) composed by 4 domains:
Beta Sheet Domain (fibronectin binding, ~17 kDa), catalytic Core Domain (Cys-His-Asp catalytic triad, Calcium-binding, GTP/GDP-binding, ~37 kDa), Beta Barrel 1 Domain (GTP/GDP-binding, ~14 kDa) and Beta Barrel 2 Domain (~12 kDa). The inactive GTP-bound enzyme is present in a closed conformation, which upon activation by Ca2+ and substrate binding opens like a pocket knife resulting in a longitudinal open conformation.
Host
Mouse
Immunogen
Human tissue transglutaminase (full length protein with N-terminal hexahistidin-tag) recombinantly produced in insect cells
Specificity
Mouse monoclonal antibody reacts with the catalytic Core Domain of TG2
(human, guinea pig, rat, mouse and dog TG2)
Specificity of A033 was determined in western blotting with human transglutaminases (TG1 – TG7, FXIII), TG2 of different species and human TG2 domains recombinantly produced in E. coli.
A033 is specific for the catalytic Core Domain of TG2. It does not cross-react with other
domains of human TG2.
A033 is specific for TG2. It does not cross-react with other human transglutaminases.
A033 recognizes human, guinea pig, rat, mouse and to a weak extend dog TG2.

A033 recognizes the epitope D438ITHTYKYPE447 (see figure in PDS). The epitope was determined using PEPperCHIP® Transglutaminase Microarray (Art. No. P111).

For more information klick here:
Poster presentation at GRC, 2016 (Girona, Spain)
Appearance
liquid
Description
Protein G-affinity purified mouse monoclonal antibody.
Reagents
75 mM NaCl, 5 mM Tris, pH7.5, 0.025% sodium azide, 50% glycerol.
Application
Western-Blotting, Immunofluorescence
Working dilutions
Optimal dilutions should be determined by the end user.
E. g. for Western-Blotting: 1 / 500 to 1 / 5,000 should be suitable
Storage
Store at -80°C.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Stable for short term at +4°C.
Delivery is possible at ambient temperature.
Reference(s)
Kanchan et al., Cell. Mol. Life Sci. 2015, 72:3009-35
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.

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