Enzyme Immuno Assay

ZediXclusive Tissue Transglutaminase EIA

Quantity Unit Price Status
1 Kit 550 € Available
Documents
Art. No.
E018
Background info
Tissue transglutaminase (tTG, TG2) displays diverse functions in various physiological processes. In addition to these physiological functions, there is strong evidence for the implication of tTG in a number of pathological conditions, including celiac disease, fibrosis, neurodegeneration and cancer. This test may be used to detect TG2 in order to explore the expression and function of tTG during (patho)physiological conditions.

TG2 EIA for quantification
Specificity
The specificity of E018 was determined with human transglutaminases: TG1 (Zedira product no. T009), TG3 (T012), TG4 (T042), TG5 (T023), TG6 (T021), TG7 (T011) and FXIII (T027).
Result: The EIA E018 is specific for TG2. It does not detect other human transglutaminases.
Specificity was further determinded with TG2 from different species: human (T002, T022, T034, T051), guinea pig (T006, T039), rat (T038), mouse (T040) and dog (T072).
Result: The EIA E018 is specific for human TG2. It does not detect TG2 from other species tested.
Assay principle
This test is based on two-site sandwich enzyme immunoassay principle. Tested specimen is placed into the microwells coated by the antibody to tissue transglutaminase (tTG). Antigen from the specimen bind coated antibody on the microwell surface. Unbound material is removed by washing procedure. Secondary antibodies directed towards tTG, and labelled with HRP, are then added into the microwells. After subsequent washing procedure, the remaining enzymatic activity bound to the microwell surface is detected and quantified by addition of chromogen-substrate mixture, stop solution and photometry at 450 nm. Optical density in the microwell is directly related to the quantity of antigen in the specimen.
Equipment
Deionised or distilled water
Graduated cylinder, 500 mL
Low protein binding tubes, clean and dry (recommended)
Pipettes for 10 and 100 μL (1- and 8-channel pipettes recommended)
Microplate washer (optional)
Microplate photometer fitted with a 450 nm filter
Dry thermostat for 37°С
ELISA evaluation program (recommended)
Application
Determination of TG2 quantity
Intended use
This kit is intended for use with biological samples. Grossly hemolytic, lipemic, or turbid samples should be avoided. Specimens may be stored short-term at 2-8°C before testing. For a longer storage, the specimens should be frozen at -20°C or lower. Repeated freezing/ thawing should be avoided.
Storage
Store the whole kit at 2 - 8°C. It is stable up to the expiry date stated on the label of the box. Do not use kit beyond its expiry date. After opening the pouch keep unused microtiter wells tightly sealed by adhesive tape (included) to minimize exposure to moisture.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

News

Blog

Events