Isopeptidase-Fluorogenic
Assays

Abz-Peptide-calibrator

Quantity Unit Price Status
10 mg 475 € Available
Documents
Art. No.
A168
Molecular Formula
Not disclosed
Molecular Weight
575 Da
Purity by HPLC
>95 % (214 nm)
Appearance
Off-white solid
Solubility
250 nM in 1% (v/v) DMSO / aqueous buffers.
Pre-dissolve the complete 10 mg (adjust to the weight given on the vial) in 348 μL DMSO to obtain a 50 mM stock solution.
DMSO stock solutions are sterile and can be stored at -20°C for at least 6 months. To avoid too many freeze-thaw cycles, we strongly recommend storage of aliquots.
Stepwise dilution: Take e.g. 10 μL of the 50 mM stock solution and dilute by adding 40 μL DMSO to obtain a 10 mM DMSO solution. Take e.g. 5 μL of the 10 mM DMSO solution and dilute by adding 1,995 μL DMSO to obtain a 25 μM DMSO solution. Finally, take e.g. 10 μL of the 25 μM DMSO solution and dilute by adding 990 μL dilution buffer to obtain your final assay concentration (250 nM).
Please adjust your calibration to your desired range by measurement of a serial dilution. According to internal assays a nanomolar range is feasible.
Application
Calibrator for fluorescent Abz-substrates. Applicable for quantification of fluorescence release, derived from quencher cleavage by transglutaminases in isopeptidase assays.
Storage
Store at -20°C, desiccate, protect from light
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

News

Blog

Events