Isopeptidase-Fluorogenic
Assays

ZediXcite 330/418 Fluorogenic FXIII-Assay Kit

Isopeptidase-activity based FXIII assay (313/418 nm)
Based on substrate A101
Quantity Unit Price Status
1 Kit 600 € Available
Documents
Art. No.
F001
Assay principle
Factor XIII is converted by Thrombin into Factor XIIIa. At the same time, Thrombin converts Fibrinogen into Fibrin. The clotting is prevented by an aggregation inhibiting peptide. Factor XIIIa cleaves a dark quenching molecule from the side chain of a modified peptide incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored on-line (excitation wavelength 313 nm; emission wavelength 418 nm). Basically, the isopeptidase activity of Factor XIIIa was described by Parameswaran et al., the modified peptide used is intellectual property of Zedira.

FXIII assay kit
Reagents in the kit
(1) SUBSTRATE REAGENT: 2 x 20 μL modified peptide dissolved in DMSO
(2) ACTIVATOR REAGENT: 2 x 100 NIH Units human Thrombin, lyophilized
(3) BUFFER REAGENT: 2 x TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, poly ethylene glycol (PEG), glycine methyl ester, clot inhibitor peptide, Heparin antagonist (hexadimethrine bromide) and sodium azide, lyophilized
Equipment
The Fluorescent Factor XIII assay can be used in standard fluorescence spectrophotometers. Refer to the instructions of the manufacturer.
Intended use
Determination of Factor XIII activity in plasma samples.
Reference(s)
Oertel et al., Anal. Biochem. 2007, 367:152-8
Sossdorf et al., Crit. Care. 2009,13:R208

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