Protein Substrates

FITC-labeled Fibrinogen

Quantity Unit Price Status
5 mg 225 € Available
Documents
Art. No.
F006
Background info
Fibrinogen (factor I) is a plasma glycoprotein that is converted by thrombin into fibrin by cleaving fibrinopeptides A+B during blood coagulation. Fibrin forms the blood clot and is subsequently cross linked by factor XIIIa. Human fibrinogen is a hexamer composed of three pairs of chains (α2β2γ2) with a molecular weight of 340 kDa.
Source
Tested human plasma from healthy donors (HBSAG, HCV and HIV-1/HIV-2 negative).
Activation
Ca2+, Thrombin
Degree of label (DOL)
≥ 6 (see Certificate of Analysis)
Appearance
yellow, solid
Solubility
at least 10 mg/ml in water
Formulation
F006 is lyophilized from a fibrinogen solution in 20 mM Tris-HCl pH 7.2, 300 mM NaCl.
Reconstitution
Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
Application
The FITC-labelled fibrinogen may be used as substrate for FXIII in a crosslinking reaction. The crosslinking of fibrin followed by fibrinolysis gives information about clot-rigidity and therefore about the degree of clot-formation catalysed by FXIII.
Storage
Storage for several months is possible at ≤ - 20°C.
Delivery is possible at ambient temperature
Reference(s)
Mutch et al., J Thromb Haemost. 2010, 8:2017-24
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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