Factor XIII

Coagulation factor XIII

(human FXIII-A2, recombinantly produced in insect cells)
Quantity Unit Price Status
100 E 725 € Available
250 E 1450 € Available
Documents
Art. No.
T197
Synonym
Recombinant fibrin stabilizing factor, protein-glutamine-γ-glutamyltransferase
Background info
100 E*, approximates 1,000 μg FXIII-A2 
250 E*, approximates 2,500 μg FXIII-A2 
*1 E is defined as the Factor XIII activity of 1 mL citrated plasma from healthy human donors.
Molecular Weight
84 kDa (monomer); 168 kDa (homodimer)
Source
Recombinantly produced in insect cells
Activation
Thrombin, Ca2+
Appearance
White solid
Description
Recombinant human Factor XIII is a homodimer (A2) composed by two chains held together by non covalent bonds. It is N-terminally fused to a hexahistidine-tag. After activation of the zymogen by Thrombin and Ca2+ to its active form (A*2, Factor XIIIa), Factor XIIIa catalyzes the formation of covalent bridges (ε-(γ-glutamyl) lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is insoluble and resistant to lysis.
Reagents
Contains TRIS buffered Human Serum Albumin (HSA), Glucose and Sodium chloride
Reconstitution
Add the volume of water specified in the certificate of analysis under Aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.
Application
Research and development purposes
Storage
Store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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