Factor XIII

Human blood coagulation Factor XIII-A2, Val34Leu-mutant

(hFXIIIVal34Leu, A subunit, recombinantly produced in insect cells)
Quantity Unit Price Status
200 µg 400 € On request
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Documents
Art. No.
T063
Synonym
Recombinant Fibrin stabilizing factor, protein-glutamine-γ-glutamyltransferase
Molecular Weight
83 kDa (monomer); 166 kDa (homodimer)
Source
Recombinantly produced in insect cells
Purity
> 95% [by SDS-PAGE]
Appearance
White lyophilized solid.
Description
Recombinant human Factor XIII-Val34Leu is a homodimer (a2) composed of two chains held together by non covalent bonds. After activation of the zymogen by Thrombin and Ca2+ to its active form (a*2, Factor XIIIa), Factor XIIIa-Val34Leu catalyzes the formation of covalent bridges (ε-(γ-glutamyl) lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is insoluble and resistant to lysis. At position 34 Valine has been replaced by Leucine.
Please note that numerotation corresponds to the wild type protein without hexahistidine-tag.
Reagents
The recombinant human Factor XIII-Val34Leu is lyophilized from 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM DTT. Sample contains maltodextrin.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
Application
rhFXIIIa-Val34Leu catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
Storage
Storage for several months is possible at ≤ - 20°C.
Delivery is possible at ambient temperature
Reference(s)
Heil et al., Thromb. Res. 2013, 131, e214–e22 
Hess et al., PLoS ONE 2012, 7(4): e35690
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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