Celiac disease antigens

Recombinant TG2 (insect cells)

Quantity Unit Price Status
250 µg 400 € Available
1 mg 1200 € Available
Documents
Art. No.
T122
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase, His6-rhTG2

human tissue transglutaminase TG2 antigen celiac disease
Molecular Weight
78 kDa
Source
Recombinantly produced in insect cells
Purity
> 95% [SDS-PAGE and Coomassie staining] 
The gel shows a single dominant protein band at ~80 kDa.
Appearance
White lyophilized solid
Reagents
The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL Maltodextrin.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
Application
The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.
Coating
Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.
Storage
T122 is stable for at least two years if stored at ≤ -20°C.
Delivery is possible at ambient temperature
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.
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