Celiac disease antigens

Recombinant human tissue transglutaminase (Cys-277-Ser) (inactive mutant)

Quantity Unit Price Status
250 µg 400 € Available
1 mg 1200 € Available
Documents
Art. No.
T246
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase
Molecular Weight
78 kDa
Source
Recombinantly produced in insect cells
Purity
HPLC: > 95% 
SDS-PAGE (Coomassie): single dominant protein band at ~80 kDa.
Appearance
White lyophilized solid.
Formulation
Purified rhTG2 is formulated in the following buffer: 
10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL Maltodextrin
Application
The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.
Coating
Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. 
Please notice that coating conditions have to be evaluated carefully.
Storage
Store at -80°C.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability data are available at -20°C, according to our overall experience stability is still given.
Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
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