Deamidated gliadin peptides

DGP and Gliadin peptides (Set No 1)

(G051, G052, G007, G054 and G055, 100 µg each)
Quantity Unit Price Status
1 Set 780 € Available
Documents
Art. No.
G056
Source
Recombinantly produced in E. coli
Purity
> 95% (SDS-PAGE and Coomassie staining)
Appearance
White lyophilized solid
Description
The set comprises the following products:
G051 26mer gliadin peptide (native 26mer gamma gliadin peptide)
G052 33mer gliadin peptide (native 33mer alpha gliadin peptide)
G007 DGPx1 (deamidated 26mer gamma gliadin peptide)
G054 33mer DGP (deamidated 33mer alpha gliadin peptide)
G055 Carrier protein control
Reagents
The recombinant proteins are lyophilized from a solution of ~50 mM NaH2PO4, pH 6.8
Reconstitution
Add the volume of H2O the proteins are lyophilized from (see Certificate of Analysis) to each vial of lyophilized powder. Rotate vials gently until solid dissolves. Further dilutions can be made in your buffer of choice. After reconstitution, the solutions should be stored frozen in working aliquots.
Application
The recombinant antigens are meant for solid (ELISA and immunoblot) and fluid phase assays as well as Western Blotting.
Coating
Dilute with your coating buffer to an appropriate concentration, e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.
Storage
Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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