Deamidated gliadin peptides

DGPx1

(deamidated gliadin peptide)
Quantity Unit Price Status
250 µg 400 € Available
1 mg 1200 € Available
Documents
Art. No.
G007
Synonym
26mer γ-gliadin, deamidated; 26mer DGP; Deamidated Gliadin Peptide – fusion protein
Molecular Weight
26 kDa
Source
Recombinantly produced in E. coli
Purity
> 95% (SDS-PAGE and Coomassie staining)
Appearance
White lyophilized solid.
Description
DGPx1 is a fusion protein of a deamidated gliadin peptide fused with a carrier protein to be used as antigen for the detection of antibodies specific for deamidated gliadin. The deamidated gliadin peptide is based on the 26-mer gamma gliadin (Dørum S. et al., J Proteome Res. 2009; 8:1748-55).
Reagents
DGPx1 is lyophilized from a solution of ~50 mM NaH2PO4, pH 6.8.
Reconstitution
Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
Application
The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.
Coating
Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.
Storage
Store at -80°C.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability dataare available at -20°C, according to our overall experience stability is still given.
Store working aliquots preferably at -80°C(if not possible at ≤ -20°C, see comment above).
Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.
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