Deamidated gliadin peptides

DGPx2

(Fusion protein of 2 deamidated gliadin peptides)
Quantity Unit Price Status
250 µg 400 € Available
1 mg 1200 € Available
Documents
Art. No.
G006
Synonym
Deamidated Gliadin Peptide – fusion protein
Molecular Weight
31 kDa
Source
Recombinantly produced in E. coli
Purity
> 95% (SDS-PAGE and Coomassie staining)
Appearance
White lyophilized solid.
Description
DGPx2 is a fusion protein of 2 different deamidated gliadin peptides, fused with a carrier protein to be used as antigen for the detection of antibodies specific for deamidated gliadin. (Dørum S. et al., J Proteome Res. 2009; 8:1748-55).
Reagents
DGPx2 is lyophilized from a solution of ~50 mM NaH2PO4, pH 6.8.
Reconstitution
Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
Application
The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.
Coating
Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.
Storage
Store at -80°C. Stability is given for at least 3 years when stored at -80°C (see retest date on Certificate of Analysis), with potential to date extend after retesting.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Solutions of DGPx2 are stable for at least 2 years when stored at ≤ -20°C.
Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above).
Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature.
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.
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