| Microbial (Pro)TGase |
| Inhibitor |
| Activity Assay Kits |
| ELISA |
| Antibodies |
| TGase Protein Labeling Kits |
EC 220.127.116.11; Protein-glutamine-γ-glutamyltransferase
AndraconTM is a brand for high quality microbial transglutaminase:
• Recombinantly produced in E. coli
• Ultra-pure and highly active
• Batch to batch consistent quality
• License free
• Produced according to an SOP in an ISO9001:2015 certified environment
AndraconTM (Microbial Transglutaminase, MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide-bound glutamine residues and a variety or primary amines.
Therefore, AndraconTM can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies.
Essentially, AndraconTM is used for the production of antibody drug conjugates (ADCs).
38334.0 Da (theoretical, activated MTG with N-terminal sequence: FRAPDSDDR...)
Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.
> 30 U/mg
50 mM HEPES pH 7.4
Labeling, immobilisation, conjugation, and modification of proteins.
Store at -80°C. Store working aliquots undiluted.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended.
Kaempffe et al., J. Pharm. Sci. 2021, S0022-3549(21)00400-7;
Früh et al., ACS Nano 2021, 15, 12161–70;
Stricker et al., J. Pediatr. Gastroenterol. Nutr. 2019, 68:e43-e50;
Spycher et al., ChemBioChem 2017, 18:1923-7;
Steffen et al., J. Biol. Chem. 2017, 292:15622-35;
Dennler et al., Chembiochem. 2015, 16:861-7;
Dennler et al., Bioconjugate Chem. 2014, 25, 569-78;
Kaufmann et al., Food Addit. Contam. Part. A 2012, 29:1364-73;
Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49:9995-7;
Gianfrani et al., Gastroenterology 2007, 133:780-9;
Pfleiderer et al., Microbiol. Res. 2005, 160:265-71;
Ando et al., Agric. Biol. Chem. 1989, 53:2613-17;
Pasternack et al., Eur. J. Biochem. 1998, 257:570-6
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.