Microbial (Pro)TGase

Andracon™ – Recombinant Microbial Transglutaminase

(HEPES-formulated, lyophilized)
Quantity Unit Price Status
25 U 270 € Available
100 U 975 € Available
250 U 2140 € Available
10 x 25 U 2140 € Available
500 U 3750 € Available
20 x 25 U 3750 € Available
Documents
Art. No.
T300
Synonym
EC 2.3.2.13; Protein-glutamine-γ-glutamyltransferase
Background info
AndraconTM is a brand for high quality microbial transglutaminase:
• Recombinantly produced in E. coli
• Ultra-pure and highly active
• Batch to batch consistent quality
• License free
• Produced according to an SOP in an ISO9001:2015 certified environment
AndraconTM (Microbial Transglutaminase, MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide-bound glutamine residues and a variety or primary amines.
Therefore, AndraconTM can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies.
Essentially, AndraconTM is used for the production of antibody drug conjugates (ADCs).
This product replaces T001 and T153.
Molecular Weight
38334.0 Da (theoretical, activated MTG with N-terminal sequence: FRAPDSDDR...)
Source
Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.
Specific Activity
> 30 U/mg
Appearance
White lyophilized solid.
Formulation
The purified transglutaminase is lyophilized from 50 mM HEPES pH 7.4.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
Application
Labeling, immobilisation, conjugation, and modification of proteins.
Storage
Store at -80°C. Upon reconstitution, store working aliquots undiluted.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended.
Reference(s)
Kaempffe et al., J. Pharm. Sci. 2021, S0022-3549(21)00400-7;
Früh et al., ACS Nano 2021, 15, 12161–70;
Stricker et al., J. Pediatr. Gastroenterol. Nutr. 2019, 68:e43-e50;
Spycher et al., ChemBioChem 2017, 18:1923-7;
Steffen et al., J. Biol. Chem. 2017, 292:15622-35;
Dennler et al., Chembiochem. 2015, 16:861-7;
Dennler et al., Bioconjugate Chem. 2014, 25, 569-78;
Kaufmann et al., Food Addit. Contam. Part. A 2012, 29:1364-73;
Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49:9995-7;
Gianfrani et al., Gastroenterology 2007, 133:780-9;
Pfleiderer et al., Microbiol. Res. 2005, 160:265-71;
Ando et al., Agric. Biol. Chem. 1989, 53:2613-17;
Pasternack et al., Eur. J. Biochem. 1998, 257:570-6
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

News

Blog

Events