Microbial (Pro)TGase

AndraconTM – recombinant microbial transglutaminase

(Production grade)
Quantity Unit Price Status
25 U 370 € Available
10 x 25 U 2950 € Available
20 x 25 U 5150 € Available
Documents
Art. No.
T153
Synonym
EC 2.3.2.13; MTG, BTG, BTGase, Protein-glutamine-γ-glutamyltransferase
Background info
AndraconTM is a brand for high quality microbial transglutaminase:
  • Recombinantly produced in E. coli
  • Ultra-pure and highly active
  • Batch to batch consistent quality
  • Scientific and technical support
  • Available from 25 U vials up to bulk amounts
  • License free
  • Produced according to an SOP in an ISO9001:2015 certified environment
  • Characterized for host cell protein, host cell DNA, and endotoxin-levels
  • Customer audits for qualification according to guideline ICH Q7 possible
Microbial Transglutaminase (MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide bound glutamine residues and a variety or primary amines.
MTG can be used to covalently cross-link proteins with each other.
Therefore, MTG can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies.
Essentially, MTG is used for the production of antibody drug conjugates (ADCs).
Molecular Weight
38,333 Da (Dispase-activated MTG with N-terminal sequence: FRAPDSDDR...)
Source
Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.
Specific Activity
> 25 U/mg
[One unit will catalyse the formation of 1 μmole of hydroxamate per min from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C, Grossowicz et al. (1950)]
Appearance
White lyophilized solid
Reagents
The purified transglutaminase is lyophilized from a solution in the following buffer: 50 mM NaOAc pH 5.0 + 0.3 M NaCl.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
Reconstituted MTG solution might be turbid. However, MTG activity is not affected. In this case, centrifuge the solution and collect the supernatant.
After reconstitution, the solution should be stored frozen in working aliquots.
Application
Labeling, immobilisation, conjugation and modification of proteins.
MTG can tolerate up to 10% DMSO in aqueous solutions without significant influence on its catalytic activity. A small activity loss of 4% was observed in the presence of 20% DMSO.
Storage
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Reference(s)
Grossowicz et al., J. Biol. Chem. 1950, 187, 111-25
Ando et al., Agric. Biol. Chem. 1989, 53, 2613-17
Pasternack et al., Eur. J. Biochem. 1998, 257, 570-6
Pfleiderer et al., Microbiol. Res. 2005, 160, 265-71
Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49, 9995-7
Kaufmann et al., Food Addit. Contam Part. A 2012, 29, 1364-73
Dennler et al., Bioconjugate Chem. 2014, 25, 569-78
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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