Microbial (Pro)TGase

Microbial transglutaminase with C-terminal His6-Tag

(MTG-His6, recombinantly produced in E. coli, gene derived from Streptomyces mobaraensis)
Quantity Unit Price Status
25 U 400 € Available
10 x 25 U 3150 € Available
20 x 25 U 5550 € Available
Documents
Art. No.
T255
Synonym
EC 2.3.2.13; Protein-glutamine-g-glutamyltransferase
Background info
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the g-carboxyamide groups of peptide-bound glutamine residues and a variety of primary amines, particularly the e-amino group of lysine. The resulting crosslink is of great significance since it is highly stable and also resistant to mechanical and proteolytic degradation.
Molecular Weight
39 kDa (activated MTG with N-terminal sequence: FRAPDSDDR...)
Source
Recombinantly produced in E. coli.
No material of animal origin is used within production process. Gene derived from Streptomyces mobaraensis.
Specific Activity
> 30 U/mg [One unit will catalyse the formation of 1 μmole of hydroxamate per min from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C, Grossowicz et al. (1950)]
Appearance
White lyophilized solid.
Reagents
The purified transglutaminase is lyophilized from 50 mM HEPES pH 7.4.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
After reconstitution, the solution should be stored frozen in working aliquots.
Application
Labeling, immobilisation, conjugation and modification of proteins.
Storage
Store at -80°C.
If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability data are available at -20°C, according to our overall experience stability is still given.
Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at -20°C, see comment above). Repeated freezing and thawing is not recommended.
Delivery is possible at ambient temperature.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
This is a replacement product for T178.

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