Factor XIII

Cynomolgus blood coagulation Factor XIII-A2

(Macaca fascicularis FXIIIA, mfFXIIIA, recombinantly produced in insect cells)
Quantity Unit Price Status
200 µg 400 € On request
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Documents
Art. No.
T161
Background info
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptide-bound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
Molecular Weight
84 kDa (monomer), 168 kDa (homodimer)
Source
Recombinantly produced in insect cells
Purity
> 95 % by SDS-PAGE under reducing conditions
Specific Activity
> 2000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after mfFXIII-A-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
White lyophilized solid.
Formulation
The purified transglutaminase is lyophilized from 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, contains maltodextrin.
Reconstitution
Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
Application
Labeling, immobilisation, conjugation and modification of proteins.
Storage
Store at -20°C in working aliquots. Repeated freezing and thawing is not recommended.
Delivery is possible at ambient temperature
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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