Factor XIII

Human Factor XIIIa, Thrombin activated

(hFXIIIa, A subunit, recombinantly produced in insect cells)
Quantity Unit Price Status
200 µg 550 € Available
Documents
Art. No.
T070
Synonym
Recombinant Fibrin stabilizing factor
Molecular Weight
78 kDa
Source
Recombinantly produced in insect cells.
Activated by human Thrombin
Purity
> 95% [by SDS-PAGE]
Appearance
White lyophilized solid.
Description
Recombinant human Factor XIII is a homodimer (a2) composed of two chains held together by non covalent bonds. After activation of the zymogen by Thrombin and Ca2+ to its active form (a*2, Factor XIIIa), Factor XIIIa catalyzes the formation of covalent bridges (ε-(γ-glutamyl) lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is insoluble and resistant to lysis.
Reagents
The recombinant activated human Factor XIIIa is lyophilized from 50 mM Tris-HCl pH 8.0. Sample contains sucrose.
Reconstitution
Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.
Application
rhFXIIIa catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
Storage
Storage for several months is possible at ≤ - 20°C.
Delivery is possible at ambient temperature
Reference(s)
Chrobok et al., PLoS One. 2018, 13:e0196433
Akbar et al., J. Med. Chem. 2017, 60:7910-27
de Jager, M. et al., Neuropathol. Appl. Neurobiol. 2016, 42, 255
Hamedani et al., Chem. Commun. 2015, 51, 1135-39
Böhm et al,. J. Med. Chem. 2014, 57:10355-65
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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