Transglutaminase 1

Human keratinocyte transglutaminase

(TG1, recombinantly produced in insect cells)
Quantity Unit Price Status
150 µg 400 € Available
Documents
Art. No.
T035
Synonym
TG1, TGase 1, keratinocyte protein-glutamine-γ-glutamyltransferase
Molecular Weight
90 kDa
Source
Recombinant, produced in Insect cells
Purity
> 90 % (visually by SDS-PAGE)
Activation
Add 10 mM Ca2+ to activate His6-rhTG1.
Activity
> 8,000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG1-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)]. 
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
White lyophilized solid
Description
His6-rhTG1 is based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHMDGPR. His6-rhTG1 is purified by IMAC to more than 90 % purity.
Reagents
The Transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.0, 10 mM Glutathion.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
Application
His6-rhTG1 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG1 may also be used for immunoprecipitation.
Storage
Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery at ambient temperature is possible
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.

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