Transglutaminase 2

Biotinylated human tissue Transglutaminase

(biotinylated hTG2, recombinantly produced in E. coli)
Quantity Unit Price Status
100 µg 400 € Available
250 µg 800 € Available
Documents
Art. No.
T106
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase
Molecular Weight
79 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)
Source
Recombinant produced in E. coli
Purity
> 95 % (visually by SDS-PAGE)
Activation
Add 10 mM Ca2+ to activate His6-rhTG2
Activity
> 750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
Liquid
Reagents
The Transglutaminase is formulated in 10 mM Sodium Phosphate pH 8.0, 15 mM NaCl. Sample contains 50% glycerol. His6-rhTG2 is a Ca2+-dependent enzyme.
Application
His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.
Storage
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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