Transglutaminase 2

Biotinylated mouse tissue transglutaminase

(Biotin-mouse TG2, recombinant in E. coli)
Quantity Unit Price Status
250 µg 550 € Available
1 mg 1650 € Available
Documents
Art. No.
T156
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
Molecular Weight
79 kDa
Source
Recombinant produced in E. coli
Purity
> 95 % (visually by SDS-PAGE)
Activation
Add 10 mM Ca2+ to activate His6-rmTG2.
Activity
> 750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rmTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)]. 
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37 °C, assay volume = 1 ml)].
Appearance
Liquid
Reagents
The Transglutaminase is formulated in 10 mM Sodium Phosphate pH 8.0, 15 mM NaCl. Sample contains 50% glycerol. His6-rmTG2 is a Ca2+-dependent enzyme.
Application
His6-rmTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. 
His6-rmTG2 may also be used for immunoprecipitation.
Storage
Store at -20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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