Transglutaminase 2

Cynomolgus tissue transglutaminase

(mfTG2, recombinantly produced in human embryonic kidney cells)
Quantity Unit Price Status
250 µg 550 € Available
Documents
Art. No.
T108
Background info
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptide-bound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
Molecular Weight
78 kDa
Source
human embryonic kidney cells (HEK-293F)
Purity
> 95 % by SDS-PAGE under reducing conditions
Specific Activity
> 1500 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after mfTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
White lyophilized solid.
Formulation
The purified transglutaminase is lyophilized from 10 mM Tris-HCl pH 7.4, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, contains maltodextrin.
Reconstitution
Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
Application
Labeling, immobilisation, conjugation and modification of proteins.
Storage
Store at -20°C in working aliquots. Repeated freezing and thawing is not recommended.
Delivery is possible at ambient temperature
Reference(s)
Protein Data Bank (PDB) entry: 3S3P (human open tTG)
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
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