Transglutaminase 2

Human tissue transglutaminase, endotoxin free

(hTG2, rec. produced in insect cells)
Quantity Unit Price Status
250 µg 450 € Available
1 mg 1350 € Available
Documents
Art. No.
T067
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
Molecular Weight
78 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)
Source
Recombinant produced in insect cells
Activation
Add 10 mM Ca2+ to activate His6-rhTG2.
Activity
> 2000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
(Frozen) liquid.
Definition
Endotoxin level: < 4.5 EU/mg, sterile filtered using 0.2 micron filter [Bacterial endotoxins according to Ph. Eur. 2.6.14.].
Formulation
The Transglutaminase is supplied in 10 mM Tris-HCl pH 7.2, 150 mM NaCl, 0.5 mM EDTA, 0.5 mM DTT, 10% Glycerol.
Application
His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation. This product is suitable for cell culture use.
Storage
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

News

Blog

Events