Transglutaminase 2

Human tissue transglutaminase, R116C mutant

(hTG2, Arg116Cys-mutant, recombinantly produced in E. coli)
Quantity Unit Price Status
1 mg 1200 € On request
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Documents
Art. No.
T173
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, protein glutamine-γ-glutamyltransferase
Background info
His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72).
It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV…. The Arg116Cys mutation was generated using site specific PCR mutagenesis. His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 90% purity.
Molecular Weight
78 kDa
Activation
The Transglutaminase is activated with 10 mM Ca2+, due to the precipitation of Calcium Phosphate a buffer exchange (e. g. Tris-Buffer) prior to activation is highly recommended.
Activity
1)> 1500 U/mg [Activity is determined by measuring the rate of fluorescence enhancementafter His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated caseinaccording to Lorand et al., Anal. Biochem. 44 (221-231).
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Caryeclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
White lyophilized solid
Reagents
The Transglutaminase is lyophilized from 10 mM NaH2PO4, 150 mM NaCl, pH 8 and less than 0.1 mM Imidazole. Sample contains maltodextrin.
Reconstitution
Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
Application
His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.
Storage
Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. 
Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature.
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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