Transglutaminase 2

Human tissue transglutaminase

(hTG2, recombinantly produced in human embryonic kidney cells )
Quantity Unit Price Status
100 µg 400 € Available
Documents
Art. No.
T034
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
Background info
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptide-bound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
Molecular Weight
78 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)
Source
Recombinant produced in human embryonic kidney cells (HEK-293F)
Purity
> 95 % (visually by SDS-PAGE)
Activation
His6-rhTG2 is a Ca2+-dependent enzyme. Add 10 mM Ca2+ to activate His6-rhTG2.
Activity
> 750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
White lyophilized solid
Description
His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72). It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEELV....
Reagents
The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 7.4, 300 mM NaCl, 1 mM EDTA, 1 mM DTT. Sample contains maltodextrin.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
Application
Labelling, immobilisation, conjugation and modification of proteins.
Storage
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. 
Delivery is possible at ambient temperature
Note
Intended for research use only, not for use in human, therapeutic or diagnostic applications.

News

Blog

Events