Transglutaminase 2

Inactive human tissue transglutaminase

(hTG2, Cys277Ser-mutant, recombinantly produced in E. coli)
Quantity Unit Price Status
250 µg 400 € Available
1 mg 1200 € Available
Documents
Art. No.
T018
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
Source
Recombinantly produced in E. coli
Purity
> 95 % (visually by SDS-PAGE)
Activity
Due to the Cys277Ser-mutation the protein has no transglutaminase activity.
Appearance
White lyophilized solid.
Description
His6-rhTG2-Cys277Ser is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72). 
It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV. At position 277 Cysteine has been replaced by Serin. Please note that numerotation corresponds to the wild type protein without hexahistidine-tag. His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.
Reagents
The Transglutaminase is lyophilized from 10 mM NaH2PO4, 150 mM NaCl, pH 8.
Reconstitution
Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.
Application
His6-rhTG2-Cys277Ser may be used for immunoprecipitation.
Storage
Store at ≤ - 20°C.
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Delivery is possible at ambient temperature
Reference(s)
Shinde et al., J. Mol. Cell Cardiol. 2018, 117:36-48
Van den Akker et al., PLoS ONE 2011, 6:e23067
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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