Transglutaminase 2

Pig tissue transglutaminase

(sTG2, recombinantly produced in E. coli)
Quantity Unit Price Status
250 µg 400 € Available
1 mg 1200 € Available
Documents
Art. No.
T159
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
Background info
sTG2 is based on the NCBI database sequence XM_003359989. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEDLIL….
sTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.
Molecular Weight
78 kDa
Purity
> 95 % (visually by SDS-PAGE)
Activation
Add 10 mM Ca2+ to activate sTG2.
Appearance
White lyophilized solid.
Reagents
The enzyme is lyophilized from 10 mM NaH2PO4, 150 mM NaCl, pH 8.0. Sample contains maltodextrin.
Reconstitution
Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution, the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
Application
sTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. sTG2 may also be used for immunoprecipitation.
Storage
Store at -20°C in working aliquots. Repeated freezing and thawing is not recommended.
Delivery is possible at ambient temperature
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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