Transglutaminase 2

Short human tissue transglutaminase, aa 1-465

(hTG2, Barrel 1 and 2 deletion-mutant, recombinantly produced in E. coli)
Quantity Unit Price Status
250 µg 400 € Available
Documents
Art. No.
T167
Synonym
Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
Molecular Weight
53.3 kDa
Source
Recombinant produced in E. coli
Activation
The Transglutaminase is activated with 10 mM Ca2+.
Important note: In order for the protein to be catalytically active, it is necessary to incubate the working solution with a reducing agent, preferably 20-30 mM DTT, for at least 30 min at room temperature, immediately before use.
Activity
Activity is determined by measuring the rate of fluorescence enhancement after rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Appearance
White lyophilized solid
Description
Short rhTG2 is a truncated variant of TG2 lacking both ß-barrel domains. The protein was expressed in E. coli based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72). It is N-terminally fused to a hexahistidine-tag. Short rhTG2 was purified by ion metal chelating chromatography (IMAC).
Reagents
The transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8 and less than 0.1 mM Imidazole. Sample contains maltodextrin.
Application
rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. rhTG2 may also be used for immunoprecipitation.
Storage
Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Reference(s)
Stamnaes et al, PLOS ONE, 2015, 10:e0134922
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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