Transglutaminase 3

Art. No.

T013

Synonym

TG3a, TGase 3a, active epidermal protein-glutamin-ε-γ-glutamyltransferase

Molecular Weight

78 kDa (52kDa + 25kDa)

Source

Recombinantly produced in insect cells.

Appearance

White lyophilized solid.

Description

Active His₆-rhTG3 is based on the TGM3-allele from clone DKFZp686J0716 (isolated from different tissues), corrected by the insertion of the missing T at Position 435. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAALGV… .
His₆-rhTG3 is produced in insect cells and purified by ion metal chelating and ion exchange chromatography to more than 95 % purity. The active Form is obtained by treatment with Dispase I. Active His₆-rhTG3 is a Ca²⁺-dependent enzyme.

Reagents

The Transglutaminase is lyophilized from 20 mM MOPS pH 6.0, 1 mM EDTA and 1 mM DTT. Sample contains maltodextrin.

Reconstitution

Add the desired volume H₂O to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage. Storage for several days is not recommended.

Application

Active His₆-rhTG3 catalyzes acyl-transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl)-lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
Active His₆-rhTG3 may also be used for immunoprecipitation and the detection of TG3 autoantibodies.

Storage

Storage for several months is possible at  - 20°C.

Reference(s)

Akbar et al., J. Med. Chem. 2017, 60:7910-27

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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