Isopeptidase-Fluorogenic
Assays

ZediXcite 330/418 Fluorogenic TG2-Assay Kit

Isopeptidase-activity based TG2 assay (330/418 nm)
Quantity Unit Price Status
1 Kit 600 € Available
Documents
Art. No.
F014
Assay principle
TISSUE TRANSGLUTAMINASE (TG2) cleaves a dark quenching molecule from the side chain of TG2 specific peptide A163 incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored (excitation wavelength 330 nm; emission wavelength 418 nm).
Reagents in the kit
(1) SUBSTRATE REAGENT (SR): 2 x 23 μL TG2 specific peptide A163 (DMSO solution).  
(2) BUFFER REAGENT (BR): 2 x 18 mL TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, glycine methyl ester (lyophilizate).  
(3) DILUTION BUFFER (DB): 1 x 18 mL TRIS buffer pH 8.1 containing sodium chloride, EDTA, DTT (lyophilizate).
Equipment
The ZediXcite Fluorogenic TG2-Assay Kit (F014) can be used in fluorescence microplate readers using microplates as well as in standard fluorescence spectrophotometers with cuvettes. Refer to the instructions of the manufacturer.
Application
Determination of TISSUE TRANSGLUTAMINASE (TG2) activity, specific and sensitive. Suitable for measurements of 1.25 nM to 10 nM (100 ng/mL to 780 ng/mL) of TG2.
Reference(s)
Parameswaran, K.N. et al. J. Biol. Chem. 1997, 272, 10311.
Oertel, K. et al. Anal. Biochem. 2007, 367, 152.

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