Antibodies and Microarrays

Antibodies are important tools in many research and diagnostic applications.
Zedira offers antibodies – polyclonal as well as monoclonal – raised against:

 

• All human transglutaminases
• Guinea pig liver transglutaminase
• Bacterial transglutaminase
• The transglutaminase reaction product: N^ε-(γ-glutamyl)-L-lysine-isopeptide bond
• Fibrinolytic enzymes: Plasmin and its zymogen (plasminogen)
• Gliadin-variants

Antibody epitope mapping
PEPperPRINT and Zedira proudly present the new PEPperCHIP^® Transglutaminase Microarray!
Standard and Custom PEPperCHIP^® Peptide Microarrays are synthesized with a unique laser printer-based method directly on-chip and are used for antibody epitope mapping, immunological research, and biomarker discovery.

Read more about PEPperCHIP^® Laser Printing Platform


Antibodies labeled with fluorescent dyes
Zedira provides many of its polyclonal antibodies with a fluorescent label like FITC or phycoerythrin or as a biotinylated version. Of course, antibodies with other tags are available on demand.

 

Indirect immunostaining
(A) Anti-TG2 (A014): mouse colon section (1:100);
(B) Anti-TG2 (A014): mouse small intestinal mucosa;
(C) Anti-TG6 (A017): astrocyte in mouse corpus callosum.

(A/B) J. Knauer, Fraunhofer Gesellschaft Leipzig; (C) A. Schulze-Krebs, S. v. Hörsten, University Erlangen


In many applications, the need for a fluorescently labeled antibody exists, ranging from fluorescence microscopy to fluorescence-activated cell sorting (FACS).

 

Direct immunostaining
(A) Transmitted light microscopy, magnification 1:40;
(B) FITC-Anti-TG2 (A028): fluorescence microscopy, magnification 1:40;
(C) FITC-Anti-TG2 (A028): fluorescence microscopy, magnification 1:20.

(A-C) W. Dieterich, University Erlangen-Nürnberg


Change Notification for Monoclonal Antibodies: 
The antibody formulation has changed in 2021 to a formulation containing 50% glycerol (Change notification antibody formulation.pdf)