Microbial TGase

Art. No.

A143

Synonym

Monoclonal antibody to microbial transglutaminase

Background info

Microbial transglutaminase (MTG or synonymous BTG for bacterial transglutaminase) was discovered by the Japanese companies Amano Enzyme® and Ajinomoto® in the late 1980ies by screening 5,000 microorganisms. The aim was the constant supply of a cheap and stable transglutaminase for food applications. The microorganism Streptomyces mobaraensis (formerly known as Streptoverticillium mobaraense) turned out to produce a calcium independent transglutaminase with the desired properties. MTG is produced as an inactive proenzyme (zymogen) with a signal sequence for its secretion to the fermentation broth. Subsequently, proteolytic cleavage of the 45 amino acid propeptide with Proteases TAMP and TAP yields active MTG (Pasternack et al., Eur J Biochem. 1998; 257:570-6, Zotzel et al., Eur J Biochem. 2003; 270:4149-55., Zotzel et al., Eur J Biochem. 2003; 270:3214-22). PreproMTG-sequence (P81453) of microbial transglutaminase from Streptomyces mobaraensis Microbial Transglutaminase crystal structures. Left: Pro-Transglutaminase (PDB-ID: 3IU0, Yang et al., J Biol Chem. 2011; 286:7301-7). The propeptide is shown in blue. Right: active Transglutaminase (PDB-ID: 1IU4, Kashiwagi et al. J Biol Chem. 2002; 277:44252-60). Active site Cysteine is marked in yellow

Host

Mouse

Clone

XM67

Immunogen

Microbial Pro-transglutaminase from Streptomyces mobaraensis, recombinantly produced in E. coli (Zedira product No. T016)

Specificity

Microbial transglutaminase*

Appearance

liquid

Description

The IgG fraction was purified by ion exchange chromatography

Formulation

75 mM NaCl, 5 mM Tris, pH7.5, 0.025% sodium azide, 50% glycerol.

Application

Western-Blotting, Immunofluorescence

Working dilutions

Optimal dilutions should be determined by the end user. E.g. for Western-Blotting or ELISA: 1 / 500 to 1 / 5,000 should be suitable

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Stable for short term at +4°C. Delivery is possible at ambient temperature.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATI ONS

Related Products

T250: Andracon® – Recombinant Microbial Transglutaminase
T300: Andracon® – Recombinant Microbial Transglutaminase
T016: Bacterial Pro-transglutaminase
T255: Microbial transglutaminase with C-terminal His₆-Tag
C102: MTG-Blocker
M001: MTG-ANiTA-KIT
Z009: ZediXclusive Microbial Transglutaminase Assay Kit
E021: Microbial Transglutaminase (MTG) ELISA
A145: Polyclonal antibody to microbial (bacterial) transglutaminase
A144: Monoclonal antibody to microbial transglutaminase