Transglutaminase 1

Art. No.

A018

Synonym

Polyclonal antibody to human keratinocyte transglutaminase (TG1)

Host

Rabbit

Immunogen

Human keratinocyte transglutaminase (full length protein with N-terminal hexahistidin-tag) recombinantly produced in insect cells

Specificity

Specificity of A018 was determined with different human transglutaminases (TG1, TG2, TG3, TG6, TG7 and FXIII) and guinea pig transglutaminase (gpTG). Figure: Equal amounts of transglutaminases were analysed by SDS-PAGE-Gel (staining with coomassie birilliant blue, left) and western blot (right, A018 diluted 1:2000 in TBST). The western blot reveals that A018 has no cross reactivity to other transglutaminases tested.

Formulation

The antibody is lyophilized from 400 µL 0.02 M Tris-HCl buffer pH 8.0, 0.028 M NaCl, 2 mg/mL human serum albumin, azide free.

Application

Western-Blotting, immuno-fluorescence, immuno-histochemistry Note: For FACS antibody A029 (FITC-labeled A018) is recommended.

Working dilutions

Optimal dilutions should be determined by the end user. E. g. for Western-Blotting: 1 / 500 to 1 / 5,000 should be suitable

Storage

Stable for a minimum of 2 years at –20°C as lyophilized powder

Reference(s)

Algarni et al., Biochem. Pharmacol. 2017, 128:55-73 Itoh et al., J. Histochem. Cytochem. 2013, 61:793-801 Fischer et al., J. Invest. Dermatol. 2013, 133:1170-7

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

A029: FITC-labeled polyclonal antibody to human keratinocyte transglutaminase (TG1)
T035: Human keratinocyte transglutaminase