Transglutaminase 2

Art. No.

A036

Synonym

Monoclonal antibody to human TG2 (Beta Barrel 1 Domain, clone XTG21)

Background info

Tissue transglutaminase is a, Ca2+-dependent enzyme (78 kDa) composed by 4 domains: Beta Sheet Domain (fibronectin binding, ~17 kDa), catalytic Core Domain (Cys-His-Asp catalytic triad, Calcium-binding, GTP/GDP-binding, ~37 kDa), Beta Barrel 1 Domain (GTP/GDP-binding, ~14 kDa) and Beta Barrel 2 Domain (~12 kDa). The inactive GTP-bound enzyme is present in a closed conformation, which upon activation by Ca2+ and substrate binding opens like a pocket knife resulting in a longitudinal open conformation (see figure). Figure: closed (left) and open (right) conformation of human tissue transglutaminase TG2 sequence deduced from TGM2 allele IMAGp958L121020

Host

Mouse

Clone

XTG21

Isotype

IgG2a (in previous PDS version given as IgG1)

Immunogen

Human tissue transglutaminase (full length protein with N-terminal hexahistidin-tag) recombinantly produced in insect cells

Specificity

Mouse monoclonal antibody binds to the Beta Barrel 1 Domain of TG2 Specificity of A036 was determined in western blotting with human transglutaminases (TG1 – TG7, FXIII), TG2 of different species and human TG2 domains recombinantly produced in E. coli.  A036 is specific for the Beta Barrel 1 Domain of TG2. It does not cross-react with other domains of human TG2.  A036 is specific for TG2. It does not cross-react with other human transglutaminases.  A036 is specific for human TG2. It does not cross-react with other TG2 from various species (human, guinea pig, rat, mouse and dog).

Appearance

liquid

Description

The IgG fraction was purified by ion exchange chromatography.

Formulation

75 mM NaCl, 5 mM Tris, pH7.5, 0.025% sodium azide, 50% glycerol.

Application

Western-Blotting, Immunofluorescence

Working dilutions

Optimal dilutions should be determined by the end user. E. g. for Western-Blotting: 1 / 500 to 1 / 5,000 should be suitable

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Stable for short term at +4°C. Delivery is possible at ambient temperature.

Reference(s)

Kanchan et al., Cell. Mol. Life Sci. 2015, 72:3009-35

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

A033: Monoclonal antibody to human TG2 (Catalytic Domain, clone XTG17)
A034: Monoclonal antibody to human TG2 (Beta Sheet Domain, clone XTG11)
A037: Monoclonal antibody to human TG2 (Beta Barrel 2 Domain, clone XTG18)