Transglutaminase 2

Art. No.

A037

Synonym

Monoclonal antibody to human TG2 (Beta Barrel 2 Domain, clone XTG18)

Background info

Tissue transglutaminase is a, Ca2+-dependent enzyme (78 kDa) composed by 4 domains: Beta Sheet Domain (fibronectin binding, ~17 kDa), catalytic Core Domain (Cys-His-Asp catalytic triad, Calcium-binding, GTP/GDP-binding, ~37 kDa), Beta Barrel 1 Domain (GTP/GDP-binding, ~14 kDa) and Beta Barrel 2 Domain (~12 kDa). The inactive GTP-bound enzyme is present in a closed conformation, which upon activation by Ca2+ and substrate binding opens like a pocket knife resulting in a longitudinal open conformation (see figure). Figure: closed (left) and open (right) conformation of human tissue transglutaminase TG2 sequence deduced from TGM2 allele IMAGp958L121020

Host

Mouse^

Clone

XTG18

Isotype

IgG3 (in previous PDS version given as IgG1)

Immunogen

Human tissue transglutaminase (full length protein with N-terminal hexahistidin-tag) recombinantly produced in insect cells

Specificity

Mouse monoclonal antibody binds to the Beta Barrel 2 Domain of TG2 Specificity of A037 was determined in western blotting with human transglutaminases (TG1 – TG7, FXIII), TG2 of different species and human TG2 domains recombinantly produced in E. coli.  A037 is specific for the Beta Barrel 2 Domain of TG2. It does not cross-react with other domains of human TG2.  A037 is specific for TG2. It does not cross-react with other human transglutaminases.  A037 is specific for human TG2. It does not cross-react with other TG2 from various species (human, guinea pig, rat, mouse and dog).

Appearance

liquid

Description

The IgG fraction was purified by ion exchange chromatography.

Formulation

75 mM NaCl, 5 mM Tris, pH7.5, 0.025% sodium azide, 50% glycerol.

Application

Western-Blotting only

Working dilutions

Optimal dilutions should be determined by the end user. E. g. for Western-Blotting: 1 / 500 to 1 / 5,000 should be suitable

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Stable for short term at +4°C. Delivery is possible at ambient temperature.

Reference(s)

Kanchan et al., Cell. Mol. Life Sci. 2015, 72:3009-35

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

A033: Monoclonal antibody to human TG2 (Catalytic Domain, clone XTG17)
A034: Monoclonal antibody to human TG2 (Beta Sheet Domain, clone XTG11)
A036: Monoclonal antibody to human TG2 (Beta Barrel 1 Domain, clone XTG21)