Transglutaminase 6

Art. No.

A158

Synonym

Monoclonal antibody to human TG6

Background info

Neuronal transglutaminase is a, Ca2+-dependent enzyme (79 kDa) composed by 4 domains: Beta Sheet Domain (~15 kDa), Catalytic Domain (Cys-His-Asp catalytic triad, ~37 kDa), Beta Barrel 1 Domain (~16 kDa) and Beta Barrel 2 Domain (~11 kDa). TG6 sequence deduced from human (TGM6 GenBank: AF540969.1) MAGIRVTKVDWQRSRNGAAHHTQEYPCPELVVRRGQSFSLTLELSRALDCEEILIFTVETGPRASEALH TKAVFQTSELERGEGWTAAREAQMEKTLTVSLASPPSAVIGRYLLSIRLSSHRKHSNRRLGEFVLLFNP WCAEDDVFLASEEERQEYVLSDSGIIFRGVEKHIRAQGWNYGQFEEDILNICLSILDRSPGHQNNPATD VSCRHNPIYVTRVISAMVNSNNDRGVVQGQWQGKYGGGTSPLHWRGSVAILQKWLKGRYKPVKYGQCWV FAGVLCTVLRCLGIATRVVSNFNSAHDTDQNLSVDKYVDSFGRTLEDLTEDSMWNFHVWNESWFARQDL GPSYNGWQVLDATPQEESEGVFRCGPASVTAIREGDVHLAHDGPFVFAEVNADYITWLWHEDESRERVY SNTKKIGRCISTKAVGSDSRVDITDLYKYPEGSRKERQVYSKAVNRLFGVEASGRRIWIRRAGGRCLWR DDLLEPATKPSIAGKFKVLEPPMLGHDLRLALCLANLTSRAQRVRVNLSGATILYTRKPVAEILHESHA VRLGPQEEKRIPITISYSKYKEDLTEDKKILLAAMCLVTKGEKLLVEKDITLEDFITIKVLGPAMVGVA VTVEVTVVNPLIERVKDCALMVEGSGLLQEQLSIDVPTLEPQERASVQFDITPSKSGPRQLQVDLVSPH FPDIKGFVIVHVATAK Sequence colour Domain monoclonal antibody recognition site Blue: Beta Sheet Domain Yellow: Catalytic Core Domain A139 Pink: Beta Barrel 1 Domain A140, A141, A157 Green: Beta Barrel 2 Domain A158 Model of structure of TG6 in ‘open’ conformation. An alignment of human TG6 and TG2, generated using CLUSTAL omega, was used to generate a homology model in SWISS model from available X-ray coordinates for human TG2 (PDB 2Q3Z) (courtesy S. Turberville and D. Aeschlimann, Cardiff University, UK

Host

Mouse

Clone

XTG29

Isotype

IgG1

Immunogen

Human neuronal transglutaminase (full length protein with N-terminal hexahistidin-tag) recombinantly produced in insect cells (Zedira product No. T021)

Specificity

β-barrel 2

Appearance

liquid

Description

The IgG fraction was purified by ion exchange chromatography.

Formulation

75 mM NaCl, 5 mM Tris, pH7.5, 0.025% sodium azide, 50% glycerol

Application

Western-Blotting, ELISA

Working dilutions

Optimal dilutions should be determined by the end user. E.g. for Western-Blotting or ELISA: 1 / 500 to 1 / 5,000 should be suitable

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. Stable for short term at +4°C. Delivery is possible at ambient temperature.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATI ONS.

Related Products

A031: FITC-labeled polyclonal antibody to human TG6
T021: Human neuronal transglutaminase