Solubility
100 μM in 0.1% (v/v) DMSO / aqueous buffers.
Pre-dissolve e.g. 5.0 mg in 53 μl DMSO (100 mM).
Take e.g. 10 μl of the 100 mM stock solution - dilute by adding 9990 μl buffer or assay components to obtain your final assay solution (100 μM).
DMSO stock solutions are sterile and can be stored at -20°C for at least 6 months.
To avoid too many freeze-thaw cycles, we strongly recommend storage of aliquots.
Reference(s)
Esposito et al. Protein Sci. 2003, 12, 1290.
Esposito et al. Am J Gastroenterol. 2003, 98, 1813.
Groenen et al. J. Biol. Chem. 1994, 14, 831.
Trigwell et al. Anal.Biochem. 2004, 330, 164.
Tseng et al. J. Cell. Biochem. 2009, 107, 899.
Ruoppolo et al. Prot. Sci. 2003, 12, 1290.
Chou et al. J. Biol. Chem. 2011, 286, 27825.
Orrù et al. J. Biol. Chem. 2003, 278, 31766.
Zindel et al. PLoS One 2016, 11, e0149145.
Recktenwald et al. J. Biol. Chem. 2016, 291, 13580