Glutamine-donor peptides

Art. No.

B001

Synonym

Biotinyl-Thr-Val-Gln-Gln-Glu-Leu-OH A25-peptide

Molecular Formula

C40H66N10O14S

Molecular Weight

943.08

Purity by HPLC

>95 % (214 nm)

Purity

>95 % (HPLC)

Appearance

White shining crystals

Solubility

100 µM in 0.1% (v/v) DMSO / aqueous buffers. Pre-dissolve e.g. 5.0 mg in 53 µl DMSO (100 mM). Take e.g. 10 µl of the 100 mM stock solution - dilute by adding 9990 µl buffer or assay components to obtain your final assay solution (100 μM). DMSO stock solutions are sterile and can be stored at -20°C for at least 6 months. To avoid too many freeze-thaw cycles, we strongly recommend storage of aliquots.

Application

Biotinylated substrate for tissue Transglutaminase

Storage

Store at -20°C, desiccate

Reference(s)

Esposito et al. Protein Sci. 2003, 12, 1290. Esposito et al. Am J Gastroenterol. 2003, 98, 1813. Groenen et al. J. Biol. Chem. 1994, 14, 831. Trigwell et al. Anal.Biochem. 2004, 330, 164. Tseng et al. J. Cell. Biochem. 2009, 107, 899. Ruoppolo et al. Prot. Sci. 2003, 12, 1290. Chou et al. J. Biol. Chem. 2011, 286, 27825. Orrù et al. J. Biol. Chem. 2003, 278, 31766. Zindel et al. PLoS One 2016, 11, e0149145. Recktenwald, C. et al. J. Biol. Chem. 2016, 291, 13580.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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