Isopeptidase-Fluorogenic Assays

Tissue Transglutaminase Assay Kit

Based on substrate A102

1 Kit 520 € available

Art. No. F002
Assay principle Tissue transglutaminase (TG2) cleaves a dark quenching molecule from the side chain of a modified peptide incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored on-line (excitation wavelength 313 nm; emission wavelength 418 nm). The modified peptide used is intellectual property of Zedira.

Tissue Transglutaminase TG2 assay
Reagents in the kit (1) SUBSTRATE REAGENT: 2 x 25 μl modified peptide dissolved in DMSO
(2) BUFFER REAGENT: 2 x 20 ml TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, glycine methyl ester, glutathion, sodium azide (0.02%, w/v).
(3) DILUTION BUFFER: 1 x 20 ml TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, sodium azide (0.02%, w/v)
Equipment The Fluorescent TG2 assay can be used in standard fluorescence spectrophotometers. Refer to the instructions of the manufacturer.
Intended use Determination of tissue transglutaminase (TG2) activity.
Storage In their original packing box, when stored at 2-8°C, the unopened reagents are stable until the expiration date printed on the box.
Reference(s) Oertel et al., Anal. Biochem. 2007, 367:152-8


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  • 19th International Celiac Disease Symposium

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