Fibrin aggregation and clotting

Fibrin Glue (porcine)



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Art. No. F007
Background info Biological two component glue from porcine plasma
Source Porcine plasma
Description Fibrin Glue is a natural product consisting of two components: Fibrinogen concentrate and thrombin. After mixing both components, polymerization of fibrin occurs resulting in an elastic and opaque fibrin matrix. During polymerization, thrombin sequentially cleaves off the fibrinopeptides A and B from fibrinogen, which normally prevent spontaneous polymerization. The result is a three-dimensional network of branching fibres that form hydrogels with large elastic moduli at very low fraction of polymer.
Reagents in the kit Component 1 (F017, Fibrinogen Conc., porcine): Frozen viscous liquid (flakes), salmon colour
Component 2 (F027, Thrombin, porcine): Frozen liquid, translucent
Application A fibrin clot is a network of proteins that is produced naturally by the blood coagulation system after vascular injury. Using purified fibrinogen and thrombin, a fibrin hydrogel can be engineered in vitro and used as a tissue substitute e.g. to simulate wound healing. The scaffold consists of naturally occurring biomaterials composed of a cross-linked network which is biodegradable. Hydrogels formed by fibrin can be manipulated to have various sizes, shapes and stiffness according to the user’s application and needs. Because of these unique properties fibrin hydrogels act as an interface for cells grown on two- or within threedimensional matrices. Additional proteins can be incorporated into fibrin hydrogels, e.g. fibronectin, vitronectin, laminin and collagen, through cross-linking by FXIII. This can be relevant to achieve a controlled release of molecules in many applications, for example in tissue culture.
Working dilutions According to the application, the ratio of component 1 to component 2 can be titrated to form hydrogels of defined shape and stiffness. A ratio of 20:1 (v/v) between component 1 and component 2 should be a useful starting point.
Storage Both components should be stored frozen at -20°C or lower
Note INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
Related Products F008 - Fibrin Glue (bovine)

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