Celiac disease antigens

Art. No.

T122

Synonym

Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase

Molecular Weight

78 kDa

Source

Recombinantly produced in insect cells

Purity

HPLC: > 95% SDS-PAGE (Coomassie): single dominant protein band at ~80 kDa

Activity

Specific activity > 2000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2 catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

Appearance

White lyophilized solid.

Formulation

Purified rhTG2 is formulated in the following buffer: 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL Maltodextrin

Reconstitution

Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.

Application

The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.

Coating

Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.

Storage

Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability data are available at -20°C, according to our overall experience stability is still given. Upon reconstitution, store undiluted working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Avoid repeated freezing and thawing. Delivery is possible at ambient temperature

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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T051: Open tTG™