Detection of gliadin antibodies has been used for a long time in celiac disease diagnostics, but suffered from a low specificity. This disadvantage was overcome by the introduction of deamidated gliadin peptides as antigen. The ratio behind is that tissue transglutaminase catalyzes gliadin deamidation in the intestinal mucosa of celiac disease patients, resulting in deamidated gliadin peptides which are recognized by HLA receptors of immune cells. Therefore deamidated gliadin antibodies are specific for celiac disease.
Our ELISAs for detection of deamidated gliadin antibodies (E019/E020) use DGPx1 as antigen. DGPx1 (G007) is our novel deamidated gliadin peptide, fused to a carrier protein and produced by recombinant techniques intended for usage as antigen in deamidated gliadin antibody detection.