Zedira's MTG-Handbook

Microbial transglutaminase (MTG) is an enzyme originally produced by Streptomyces species. MTG catalyzes cross-linking of proteins in the absence of calcium. In simple words: MTG is a biological glue. While its physiological role is still not fully understood, MTG received major attraction by the industry for its protein cross-linking activity. It is applied in the production processes of meat, sausage, dairy, bakery, and pasta products to improve food properties.

Moreover, MTG cannot only cross-link proteins, but it can also incorporate primary amines to proteins in a covalent, irreversible manner. This feature allows to link a whole bundle of labels to proteins, including biotin, fluorescent dyes, click chemistry substrates, and cytotoxins.

MTG attracted more and more interest, as developers of antibody drug conjugates realized its unique property to generate site specific and homogenous antibody drug conjugates (ADCs). Today, ADCs manufactured with MTG entered clinical trials.

Zedira’s MTG-Handbook shall provide an overview on this fascinating enzyme. We highly appreciate the blogs of renowned colleagues form the MTG community as part of the handbook. This first edition is just the beginning – future editions will follow to include latest developments and to cover novel applications.

View of Table of Content:
  1. Transglutaminases
  2. MTG – Structure and Numbers.pdf
  3. Biology of Microbial Transglutaminase
  4. Enzymology
  5. The Food Enzyme
  6. The Technical Enzyme
  7. Site Specific Conjugation - ADCs and More.pdf
  8. How to Measure and Detect MTG
  9. MTG-Products and Tools
  10. Application Notes
  11. References

Please feel free to contact us if you would like to receive your personal hard copy of our handbook: contact@zedira.com


  • Zedira offers a huge portfolio of reliable specialist transglutaminase related products meant to catalyze research and development, both in academia and industry.


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  • Gordon Research Conference: Transglutaminases in Human Disease Processes

    12.06.2022 - 17.06.2022
    Mount Snow, USA

  • 19th International Celiac Disease Symposium

    19.10.2022 - 22.10.2022
    Sorrento, Italy