Zedira's MTG-Handbook
Microbial transglutaminase (MTG) is an enzyme originally produced by the
Streptomyces species. MTG catalyzes cross-linking of proteins in the absence of calcium. In simple words: MTG is a biological glue. While its physiological role is still not fully understood, MTG is primarily known by the industry for its protein cross-linking activity. It is applied in the production processes of meat, sausage, dairy, bakery, and pasta products to improve food properties.
Moreover, MTG can not only cross-link proteins, but can also incorporate primary amines into proteins in a covalent, irreversible manner. This feature allows linking a bundle of labels to proteins, including biotin, fluorescent dyes, click chemistry substrates, and cytotoxins.
MTG attracted more interest as developers of antibody-drug conjugates realized its unique property to generate site-specific and homogenous antibody-drug conjugates (ADCs). Today, ADCs manufactured with MTG have entered clinical trials.
Zedira’s MTG-Handbook provides an overview of this fascinating enzyme. We highly appreciate the blogs of renowned colleagues from the MTG community as part of the handbook. The 2
nd edition of Zedira’s MTG-Handbook includes additional information on MTG’s characteristics useful for everyday work in the lab: pH- and temperature profile, stability, and more. In addition, a starting protocol for antibody conjugation is included.
Download your softcopy here!