Zedira's MTG-Handbook


Microbial transglutaminase (MTG) is an enzyme originally produced by the Streptomyces species. MTG catalyzes cross-linking of proteins in the absence of calcium. In simple words: MTG is a biological glue. While its physiological role is still not fully understood, MTG is primarily known by the industry for its protein cross-linking activity. It is applied in the production processes of meat, sausage, dairy, bakery, and pasta products to improve food properties. 

Moreover, MTG can not only cross-link proteins, but can also incorporate primary amines into proteins in a covalent, irreversible manner. This feature allows linking a bundle of labels to proteins, including biotin, fluorescent dyes, click chemistry substrates, and cytotoxins. 

MTG attracted more interest as developers of antibody-drug conjugates realized its unique property to generate site-specific and homogenous antibody-drug conjugates (ADCs). Today, ADCs manufactured with MTG have entered clinical trials. 

Zedira’s MTG-Handbook provides an overview of this fascinating enzyme. We highly appreciate the blogs of renowned colleagues from the MTG community as part of the handbook. The 2nd edition of Zedira’s MTG-Handbook includes additional information on MTG’s characteristics useful for everyday work in the lab: pH- and temperature profile, stability, and more. In addition, a starting protocol for antibody conjugation is included. 

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