Activity Assay Kits

Art. No.

F015

Synonym

ZediXcite 330/418 Fluorogenic MTG-Assay Kit

Description

Isopeptidase-activity based ready to use kit for fluorescent measurement of Microbial Transglutaminase (MTG or synonymous BTG for Bacterial Translutaminase). MTG cleaves a dark quenching molecule (2,4-dinitrophenyl) from the side chain of a modified peptide incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled fluorophore (2-amino benzoic acid, 2-Abz) increases and can be monitored online (excitation wavelength 313-330 nm; emission wavelength 418 nm). The assay is suitable for research purposes, drug discovery programs, and quality assurance

Assay principle

MICROBIAL TRANSGLUTAMINASE (MTG) cleaves a dark quenching molecule from the side chain of MTG specific peptide A167 incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored (excitation wavelength 330 nm; emission wavelength 418 nm).

Reagents in the kit

(1) SUBSTRATE REAGENT (SR): 2 x 23 μL MTG specific peptide A167 (DMSO solution).
(2) BUFFER REAGENT (BR): 2 x 18 mL TRIS buffer pH 6.0 containing glycine methyl ester (lyophilizate).
(3) DILUTION BUFFER (DB): 1 x 18 mL TRIS buffer pH 6.0 (lyophilizate).

Equipment

The ZediXcite Fluorogenic MTG-Assay Kit (F015) can be used in fluorescence microplate readers using microplates as well as in standard fluorescence spectrophotometers with cuvettes. Refer to the instructions of the manufacturer.

Application

Determination of MICROBIAL TRANSGLUTAMINASE (MTG) activity, specific and sensitive. Suitable for measurements of 2.5 nM to 80 nM (0.1 μg/mL to 3 μg/mL) of MTG.

Storage

Store at 2-8 °C.

Reference(s)

Parameswaran, K.N. et al. J. Biol. Chem. 1997, 272, 10311.
Oertel, K. et al. Anal. Biochem. 2007, 367, 152.

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

Related Products

T250: Andracon® – Recombinant Microbial Transglutaminase
T300: Andracon® – Recombinant Microbial Transglutaminase
F014: ZediXcite 330/418 Fluorogenic TG2-Assay Kit