Andracon®

Art. No.

T300

Synonym

Protein-glutamine-γ-glutamyltransferase (Enzyme Commission No 2.3.2.13)

Background info

Andracon® is a brand for high quality microbial transglutaminase: • Recombinantly produced in E. coli • Ultra-pure and highly active • Batch to batch consistent quality • License free • Produced according to an SOP in an ISO9001:2015 certified environment Andracon® (Microbial Transglutaminase, MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide-bound glutamine residues and a variety or primary amines. Therefore, Andracon® can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies. Essentially, Andracon® is used for the production of antibody drug conjugates (ADCs).

Molecular Weight

38333.6 Da

Source

Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.

Specific Activity

> 30 U/mg

Appearance

White lyophilized solid.

Formulation

The purified transglutaminase is lyophilized from a solution in 50 mM HEPES pH 7.4.

Reconstitution

Add the volume of pure water the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.

Application

Labeling, immobilisation, conjugation, and modification of proteins. MTG reaction pathway for labeling of (deglycosylated) antibodies. Antibody heavy chains are conjugated with a drug (linked to a primary amine) by MTG on position Q295, resulting in an ADC with site specifically conjugated drug molecules. Here, conjugation of only one Q295 is shown.

Storage

Store at -80°C ± 5°C. Exceeding short-time temperature increases resulting from the -80°C freezer’s handling are acceptable. If storage at -80°C is not possible, storage at ≤ -20°C is recommended, see section “stability”. Upon reconstitution, store working aliquots undiluted. Delivery is possible at ambient temperature. Stability of lyophilized Andracon® is given for at least 4 weeks at up to 37 °C.

Reference(s)

Kaempffe et al., J. Pharm. Sci. 2021, S0022-3549(21)00400-7; Früh et al., ACS Nano 2021, 15, 12161–70; Stricker et al., J. Pediatr. Gastroenterol. Nutr. 2019, 68:e43-e50; Spycher et al., ChemBioChem 2017, 18:1923-7; Steffen et al., J. Biol. Chem. 2017, 292:15622-35; Dennler et al., Chembiochem. 2015, 16:861-7; Dennler et al., Bioconjugate Chem. 2014, 25, 569-78; Kaufmann et al., Food Addit. Contam. Part. A 2012, 29:1364-73; Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49:9995-7; Gianfrani et al., Gastroenterology 2007, 133:780-9; Pfleiderer et al., Microbiol. Res. 2005, 160:265-71; Ando et al., Agric. Biol. Chem. 1989, 53:2613-17; Pasternack et al., Eur. J. Biochem. 1998, 257:570-6

Note

Always obtain, read, and observe the material safety data sheet (MSDS) precautions prior to handling or using this product. No testing has been conducted to determine the suitability of this product for use in or on humans or animals or for in vitro diagnostic applications (e.g., in or as food, consumer goods, feed materials, cosmetics, medicinal products, medical devices). Thus, this product is not approved for any such use. If any such use of this product is intended by customer, customer will be solely responsible to obtain any necessary approvals and to meet any other requirements under applicable laws and regulations associated with such intended use.

Related Products

T250: Andracon® – Recombinant Microbial Transglutaminase