Pro-MTG

Bacterial Pro-transglutaminase

(Pro-BTG, recombinantly produced in E. coli
gene derived from Streptomyces mobaraensis)
Quantity Unit Price Status
250 µg 330 € Available
Documents
Art. No.
T016
Synonym
Protein-glutamine-γ-glutamyltransferase; Bacterial Pro-transglutaminase, pro-MTG, pro-BTG
Molecular Weight
43 kDa
Source
Recombinantly produced in E. coli
Purity
> 90% [SDS-PAGE and Coomassie staining]
Activation
10 μl His6-rProTG (1 μg/μl) 35 μl 50 mM Tris-HCl pH 8 5 μl Dispase II (Roche) (0.2 μg/μl) Incubate for 20 min at 30°C
Specific Activity
13 U/mg [One unit will catalyse the formation of 1 μmole of hydroxamate per min from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C, Grossowicz et al. (1950)]
Appearance
White lyophilized solid.
Reconstitution
Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. For short term storage keep cooled on ice.
Application
Labeling, immobilisation, conjugation and modification of proteins.
Storage
Store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. Delivery at ambient temperature is possible
Reference(s)
Pasternack et al., Eur. J. Biochem. 1998, 257:570-6
Note
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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